INNOVATIVE IDEAS FOR RAM SEMEN FREEZING INNOVATIVE IDEAS FOR RAM SEMEN FREEZING c. EFFECT OF EXTENDER COMPOSITION, COOLING RATE, AND THAWING METHOD ON RAM SEMEN

Document Type : Original Article

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1 teacher

2 Animal Production Department, Faculty of Agriculture, Minufia Universit, Egypt.

3 قسم الانتاج الحيوانى كلية الزراعة جامعة المنوفية

Abstract

Two experiments were implemented in this investigation.  Semen was collected from four adult Ossimi rams using an artificial vagina in the two experiments (V and VI). Ejaculates were pooled together and gently mixed to study; effect of two cooling rates of the fresh semen were examined i.e., (fast cooling: cooling from 30-32°C to 15°C over 10 minutes versus slow cooling: cooling from 30-32°C to 15°C over 30 minutes), and three thawing rates (T1, T2 and T3) were tested: T1 involved thawing frozen straws at 42 °C for 35 seconds; T2 consisted of thawing frozen straws at 100 °C for 4 seconds, followed by 42 °C for 5 seconds; T3 consisted of thawing frozen straws at 100 °C for 5 seconds, followed by 42 °C for 5 seconds.
In the previous experiment IV, two extenders, A-extender and C-extender, were used. All processing, semen evaluation, and statistical analysis were the same.
The results from experiment V indicated that the fast-cooling rate significantly affected the motility and viability percentages of ram spermatozoa after glycerolation, equilibration, and post–thawing compared to slow cooling. The extender effect on both sperm motility and viability percentages was also significant after glycerolation (P<0.05) and equilibration (P<0.01), and insignificantly on post–thawing. The final results of post-thawing motility and livability were (66.9 and 65.8%, respectively) for A-extender and (64.4 and 64.3%, respectively) for C-extender

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