INNOVATIVE IDEAS FOR RAM SEMEN FREEZING a. EFFECT OF EXTENDER COMPOSITION, ADDITION METHOD OF EXTENDER AND TEMPERATURE AT ADDITION EXTENDER ON RAM SEMEN FREEZABILITY

Document Type : Original Article

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1 teacher

2 Animal Production Department, Faculty of Agriculture, Minufia Universit, Egypt.

3 قسم الانتاج الحيوانى كلية الزراعة جامعة المنوفية

Abstract

Semen was collected from four adult Ossimi rams using an artificial vagina to study the effects of the extender addition either on one-step or two-step technique, with three temperatures at extender addition to raw semen at (25°C, 15°C and 5°C) on ram semen freezability in liquid Nitrogen (-196°C). Two new extenders were used, Tris-based extender (A-extender) and Tris-based extender containing 35% skim milk (B-extender). The final glycerol level was 14.5% in two extenders, and the dilution rate was 1:6 (v/v). The equilibration period was 3 hours. The frozen semen was thawed at 42°C for 35 seconds. Sperm motility and livability were determined to evaluate semen at various freezing and thawing stages.
A-extender yielded the best post-thaw motility and livability (44.4 and 43.8 %, respectively) compared with B-extender, which recorded 33.1 and 32.7% values for motility and livability, respectively.
One-step method yielded better values (P<0.01) of post-thaw motility and livability as 40.4 and 40.0 %, respectively, compared with the two-step method, which revealed 37.2 and 36.5% values, respectively. Addition of extender at 15°C yielded better results (P<0.01) of motility and livability than addition at 25°C and significantly better than 5°C.
It could be advisable to use A-extender added to raw ram semen in one step at 15°C to improve semen freezability.

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